分析小鼠外周血、脾脏中T、B、NK、单核、粒细胞、巨噬细胞、DC等细胞群体的比例及数量,评估小鼠的免疫系统。小鼠经过基因敲除、靶点改造、药物诱导后,免疫细胞会发生改变,通过流式分析各类免疫细胞,寻找基因或药物影响的细胞群体,有助于基因及药物的功能研究。
实验案例:BALB/c小鼠外周血分析12色方案
Population | Gating Step | ||||
Total Leukocytes | Live Single | mCD45+ | / | / | / |
Neutrophils | Live Single | mCD45+ | mCD11b+ | Ly6G+ | / |
Monocytes | Live Single | Not Neutrophils | mCD11b+ | Ly6C hi | / |
Eosinophils | Live Single | Not Monocytes | mCD11b+ | SSC-H hi | / |
Macrophages | Live Single | Not Eosinophils | mCD11b+ | F4/80+ | / |
DC Cells | Live Single | Not Eosinophils | mCD11b+ | mCD11c+ | / |
T Cells | Live Single | Not Eosinophils | mCD3+ | mCD335- | / |
B Cells | Live Single | Not Eosinophils | mCD19+ | / | / |
NK Cells | Live Single | Not Eosinophils | mCD3- | mCD335+ | / |
Th Cells | Live Single | Not Eosinophils | mCD3+ | mCD4+ | mCD8- |
Tc Cells | Live Single | Not Eosinophils | mCD3+ | mCD4- | mCD8+ |
南宫28ng优势:
1. 拥有丰富的免疫细胞分析检测经验,满足血样、脾脏、淋巴结、骨髓等样品分析,实验数据稳定;
2. 提供常规免疫分群检测Panel,同时可以根据小鼠特点,针对性的检测独特的细胞群体及稀有群体,提供定制化服务;
3. 严格的实验室数据保存方案,实验数据具有可追溯性 ,满足新药申报合规性要求。